- Revamped Enzyme List and Enzyme Selection Criteria
- New list composed of prototypes (and their neoschizomers) that are commercially available, single cutting, Type IIs with known cleavage sites.
- No longer use buffer, vendor, and NEB availability as criteria.
- Added incubation temperature, methylation sensitivity, star activity and exhaustive vendor list.
- Now able to exclude enzymes with cleavage external to recognition site.
- Added C. elegans to list of known organisms
- Oligo Design now automatically excludes blunts, 1bp overhangs, and enzymes that cut outside of recognition site.
- Codon Juggling detailed
- Now warns if you are not using a simple coding sequence
- Will allow you to not define an organism; hides optimization algorithms and randomizes most different
- Define next most optimal and random as highly experimental algorithms
- Silent Site Insertion detailed
- Allow reconsideration of automatic site selection
- Fixed error messages about simple coding sequences
- Miscellaneous bug fixes that you probably never noticed
- Manual updated accordingly and PDF version added.
- Now with version history!
Changes from 1.9 to 2.0