AarI (CACCTGC)
AatII (GACGTC)
AccI (GTMKAC)
AciI (CCGC)
AclI (AACGTT)
AcyI (GRCGYC)
AflII (CTTAAG)
AflIII (ACRYGT)
AgeI (ACCGGT)
AluI (AGCT)
AlwI (GGATC)
AlwNI (CAGNNNCTG)
ApaI (GGGCCC)
ApaLI (GTGCAC)
ApoI (RAATTY)
AscI (GGCGCGCC)
AsuII (TTCGAA)
AvaI (CYCGRG)
AvaII (GGWCC)
AvrII (CCTAGG)
BalI (TGGCCA)
BamHI (GGATCC)
BanI (GGYRCC)
BanII (GRGCYC)
BbeI (GGCGCC)
BbsI (GAAGAC)
BbvCI (CCTCAGC)
BbvI (GCAGC)
BccI (CCATC)
BceAI (ACGGC)
BciVI (GTATCC)
BclI (TGATCA)
BfrBI (ATGCAT)
BglI (GCCNNNNNGGC)
BglII (AGATCT)
BisI (GCNGC)
BlpI (GCTNAGC)
Bpu10I (CCTNAGC)
BpuEI (CTTGAG)
BsaAI (YACGTR)
BsaBI (GATNNNNATC)
BsaJI (CCNNGG)
BsaWI (WCCGGW)
BseMII (CTCAG)
BseRI (GAGGAG)
BseSI (GKGCMC)
BseYI (CCCAGC)
BsgI (GTGCAG)
BsiEI (CGRYCG)
BsiHKAI (GWGCWC)
BsiYI (CCNNNNNNNGG)
BsmAI (GTCTC)
BsmI (GAATGC)

Bsp120I (GGGCCC)
Bsp1407I (TGTACA)
BspEI (TCCGGA)
BspHI (TCATGA)
BspLU11I (ACATGT)
BspMI (ACCTGC)
BsrBI (CCGCTC)
BsrDI (GCAATG)
BsrI (ACTGG)
BssHII (GCGCGC)
BssSI (CACGAG)
Bst1107I (GTATAC)
BstAPI (GCANNNNNTGC)
BstEII (GGTNACC)
BstF5I (GGATG)
BstNI (CCWGG)
BstUI (CGCG)
BstXI (CCANNNNNNTGG)
Bsu36I (CCTNAGG)
BtgI (CCRYGG)
BtgZI (GCGATG)
BtrI (CACGTC)
BtsI (GCAGTG)
Cac8I (GCNNGC)
Cfr10I (RCCGGY)
CfrI (YGGCCR)
ClaI (ATCGAT)
CviAII (CATG)
DdeI (CTNAG)
DpnI (GATC)
DraI (TTTAAA)
DraII (RGGNCCY)
DraIII (CACNNNGTG)
DrdI (GACNNNNNNGTC)
EagI (CGGCCG)
Eam1105I (GACNNNNNGTC)
EciI (GGCGGA)
Eco31I (GGTCTC)
Eco47III (AGCGCT)
Eco57I (CTGAAG)
Eco57MI (CTGRAG)
EcoICRI (GAGCTC)
EcoNI (CCTNNNNNAGG)
EcoRI (GAATTC)
EcoRII (CCWGG)
EcoRV (GATATC)
EcoT22I (ATGCAT)
Esp3I (CGTCTC)
FatI (CATG)
FauI (CCCGC)
Fnu4HI (GCNGC)
FokI (GGATG)
FseI (GGCCGGCC)

FspAI (RTGCGCAY)
FspI (TGCGCA)
GsuI (CTGGAG)
HaeII (RGCGCY)
HaeIII (GGCC)
HgaI (GACGC)
HhaI (GCGC)
HinP1I (GCGC)
HindII (GTYRAC)
HindIII (AAGCTT)
HinfI (GANTC)
HpaI (GTTAAC)
HpaII (CCGG)
HphI (GGTGA)
Hpy188I (TCNGA)
Hpy188III (TCNNGA)
Hpy99I (CGWCG)
HpyCH4III (ACNGT)
KasI (GGCGCC)
KpnI (GGTACC)
Ksp632I (CTCTTC)
MaeI (CTAG)
MaeII (ACGT)
MaeIII (GTNAC)
MboI (GATC)
MboII (GAAGA)
MfeI (CAATTG)
MluI (ACGCGT)
MlyI (GAGTC)
MmeI (TCCRAC)
MnlI (CCTC)
MseI (TTAA)
MslI (CAYNNNNRTG)
MspA1I (CMGCKG)
MwoI (GCNNNNNNNGC)
NaeI (GCCGGC)
NarI (GGCGCC)
NciI (CCSGG)
NcoI (CCATGG)
NdeI (CATATG)
NheI (GCTAGC)
NlaIII (CATG)
NlaIV (GGNNCC)
NotI (GCGGCCGC)
NruI (TCGCGA)
NspI (RCATGY)
OliI (CACNNNNGTG)
PacI (TTAATTAA)
PflMI (CCANNNNNTGG)
PfoI (TCCNGGA)
PleI (GAGTC)
PmaCI (CACGTG)
PmeI (GTTTAAAC)

PpuMI (RGGWCCY)
PshAI (GACNNNNGTC)
PsiI (TTATAA)
PspXI (VCTCGAGB)
PstI (CTGCAG)
PvuI (CGATCG)
PvuII (CAGCTG)
RsaI (GTAC)
RsrII (CGGWCCG)
SacI (GAGCTC)
SacII (CCGCGG)
SalI (GTCGAC)
SanDI (GGGWCCC)
SapI (GCTCTTC)
Sau96I (GGNCC)
ScaI (AGTACT)
ScrFI (CCNGG)
SduI (GDGCHC)
SexAI (ACCWGGT)
SfaNI (GCATC)
SfcI (CTRYAG)
SfiI (GGCCNNNNNGGCC)
SgfI (GCGATCGC)
SgrAI (CRCCGGYG)
SmaI (CCCGGG)
SmlI (CTYRAG)
SnaBI (TACGTA)
SpeI (ACTAGT)
SphI (GCATGC)
SrfI (GCCCGGGC)
Sse8387I (CCTGCAGG)
SspI (AATATT)
StuI (AGGCCT)
StyI (CCWWGG)
SwaI (ATTTAAAT)
TaiI (ACGT)
TaqI (TCGA)
TatI (WGTACW)
TauI (GCSGC)
TfiI (GAWTC)
TseI (GCWGC)
Tsp45I (GTSAC)
TspEI (AATT)
TspRI (CASTGNN)
Tth111I (GACNNNGTC)
VspI (ATTAAT)
XbaI (TCTAGA)
XcmI (CCANNNNNNNNNTGG)
XhoI (CTCGAG)
XhoII (RGATCY)
XmaI (CCCGGG)
XmnI (GAANNNNTTC)
ZraI (GACGTC)