The Design a Gene pathway is designed for the simple and rapid generation of oligos encoding a synthetic gene from a protein sequence.
GeneDesign will perform stepwise modifications to an amino acid sequence to produce a
codon-optimized nucleotide sequence that codes for the specified protein. The modified
nucleotide sequence can have dozens of customizations that will allow users to proceed
to complex experimental work designed to quickly elucidate the structure and function
of the gene in question. The final nucleotide sequence will be comprised of segments
linked by restriction sites, allowing users to swap synthetic segments with native
segments or to easily introduce mutations, in order to help determine which regions
are necessary for the function of the gene.
The first module in the pathway is Reverse Translation, followed by Silent
Site Insertion and Oligo Design.